What you'll learn
Human Genome project
Genome sequencing
High throughput sequencing
National Genome sequencing
Whole Genome sequencing
genetic modifications
Requirements
All life science students
Description
Part 1: Introductionshot gun experiment explanation. Cosmid DNA. sonication. random fragments.Part 2: Applications, Human Genome project, Genome sequencing, whole Genome sequencing and genetic modification.part 3:Gene library Genomic library a) Nuclear Genomic library b) Organelle Genomic library Construction of Genomic library Screening of genomic library Diagram explanation colony hybridization plaque hybridizationapplication of genomic librarystorage of genomic librarypart 4:CDNA libraryDiagram and definitionconstruction of CDNA library isolation mRNA CDNAs into vectorsuitable hostscreeningpart 5:CDNA library continues and applicationsDisadvantages and Genomic library vs CDNA library.strategies:Cancer, including genitourinary malignancy, is a consequence of accumulated genetic aberrations in genes involved in crucial regulatory pathways. The result is a deregulation of cellular behavior, leading to neoplastic transformation, uncontrolled cell proliferation and acquisition of metastatic ability. The development and perfection of techniques in the field of recombinant DNA technology, gene cloning, (differential) analysis of gene expression, and sequencing of genes and proteins have provided a wealth of information about the genetic aberrations associated with cancer development. This "recombinant DNA and gene cloning" technology and the recently developed DNA chip technology may provide new molecular diagnostic tools. Furthermore, the technology of gene cloning in combination with the progress in in vivo gene delivery techniques offers new treatment modalities, like gene therapy, additional to conventional therapies. This review is intended to provide a general introduction to the fundamentals or strategies of recombinant DNA and gene cloning techniques as a basis for understanding the rapidly expanding range of new diagnostic and therapeutic opportunities. Some illustrative examples are provided, addressing basic and biomedical research and possible clinical applications in genitourinary oncology.
Overview
Section 1: Introduction
Lecture 1 Introduction
Lecture 2 Cloning strategies-2
Lecture 3 Cloning strategies-3
Lecture 4 Cloning strategies-4
Lecture 5 Cloning strategies-5
Bsc Bio-technoligy,B pharmacy,M pharmacy,Msc Biotechnology,Msc Mircobiology,Msc Biochemistry,Medical students
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